PointSSC: A Cooperative Vehicle-Infrastructure Point Cloud Benchmark for Semantic Scene Completion

Semantic Scene Completion (SSC) aims to jointly generate space occupancies and semantic labels for complex 3D scenes. Most existing SSC models focus on volumetric representations, which are memory-inefficient for large outdoor spaces. Point clouds provide a lightweight alternative but existing benchmarks lack outdoor point cloud scenes with semantic labels. To address this, we introduce PointSSC, the first cooperative vehicle-infrastructure point cloud benchmark for semantic scene completion. These scenes exhibit long-range perception and minimal occlusion. We develop an automated annotation pipeline leveraging Segment Anything to efficiently assign semantics. To benchmark progress, we propose a LiDAR-based model with a Spatial-Aware Transformer for global and local feature extraction and a Completion and Segmentation Cooperative Module for joint completion and segmentation. PointSSC provides a challenging testbed to drive advances in semantic point cloud completion for real-world navigation.Comment: 8 pages, 5 figures, submitted to ICRA202

Research of Seepage Flow Law of Tight Oil Reservoir in North Songliao Basin

Tight oil resources in north Songliao basin is rich and abundant, which is the most important energy sources foundation of Stable and raising oil production in Daqing oilfield. However, it is difficult to develop such oil resources by the regular ways and has no economic benefit for the poor reservoir property and thin reservoir thickness. Using the way of horizontal well by volume fracturing can reach the high oil production, which has showed good results up till now. But now tight oil in Daqing oilfield exploration and development is still in its infancy, the pore structure and seepage flow law of tight oil reservoir in north Songliao basin is not clear, which is potential barrier for exploring tight oil reservoirs effective development mode. Tight oil reservoir single phase and two phase fluid seepage rule are researched by means of theoretical and experimental, the results of which can provides a reliable theoretical basis for tight oil reservoir developed effectively

Productivity Evaluation Method of Horizontal Well Volume Fracturing in Tight Oil Reservoir

Tight oil resources in north Songliao basin is rich and abundant, which is the most important energy sources foundation of stable and raising oil production in Daqing oil field. However, it is difficult to develop such oil resources by the regular ways for the poor reservoir property and thin reservoir thickness. Using the way of horizontal well by volume fracturing can increase contract area of well and the reservoir, improve reservoir flow performance and reach the high oil production, which has showed good results up till now. The accurate productivity evaluation of volume fracturing horizontal well is an important content of reservoir and production engineering field, which is also to develop solutions and decision-making basis. The current formula of horizontal well in low permeability reservoirs production did not consider the effect of seepage volume form fracturing, so it is poorly adapt to calculate the productivity of volume fracturing horizontal well. Based on the tight oil reservoir geological characteristics and seepage characteristics, equation are solved coupling with flow through fractures in the substrate, productivity prediction model is established and the innovation is based on considering horizontal well reservoir heterogeneity, fracturing scale and any artificial fracture distribution form, the results of which can provides a reliable theoretical basis for tight oil reservoir developed effectively

Phosphorylation of the chromatin binding domain of KSHV LANA.

The Kaposi sarcoma associated herpesvirus (KSHV) latency associated nuclear antigen (LANA) is expressed in all KSHV associated malignancies and is essential for maintenance of KSHV genomes in infected cells. To identify kinases that are potentially capable of modifying LANA, in vitro phosphorylation assays were performed using an Epstein Barr virus plus LANA protein microarray and 268 human kinases purified in active form from yeast. Interestingly, of the Epstein-Barr virus proteins on the array, the EBNA1 protein had the most similar kinase profile to LANA. We focused on nuclear kinases and on the N-terminus of LANA (amino acids 1-329) that contains the LANA chromatin binding domain. Sixty-three nuclear kinases phosphorylated the LANA N-terminus. Twenty-four nuclear kinases phosphorylated a peptide covering the LANA chromatin binding domain (amino acids 3-21). Alanine mutations of serine 10 and threonine 14 abolish or severely diminish chromatin and histone binding by LANA. However, conversion of these residues to the phosphomimetic glutamic acid restored histone binding suggesting that phosphorylation of serine 10 and threonine 14 may modulate LANA function. Serine 10 and threonine 14 were validated as substrates of casein kinase 1, PIM1, GSK-3 and RSK3 kinases. Short-term treatment of transfected cells with inhibitors of these kinases found that only RSK inhibition reduced LANA interaction with endogenous histone H2B. Extended treatment of PEL cell cultures with RSK inhibitor caused a decrease in LANA protein levels associated with p21 induction and a loss of PEL cell viability. The data indicate that RSK phosphorylation affects both LANA accumulation and function

LANA protein loss is post-transcriptional and leads to p21 induction.

<p>Comparison of LANA protein (A,B) and mRNA levels (C) with p21 mRNA induction (D) in BC3 and BCBL1 PEL cells treated for 1, 2 or 3 days with 1.7 µM BRD 7389. Protein levels were quantified using Image J and RNA was quantified using real time RT-PCR.</p

Phosphomimetic mutations of LANA S10 and T14 rescue histone binding.

<p>A. Western blots showing the effect on binding to histone H2B of individual alanine substitutions across the LANA chromatin binding domain. Flag-LANA was immunoprecipitated from transfected HEK293T cells and the bound endogenous H2B was detected using anti-H2B antibody. B. Western blots showing the effect of individual and grouped phosphomimetic mutations at S10, S13 and T14 on LANA binding to H2B. Flag-LANA or Flag-LANA deleted for the central repeats (dCR) was immunoprecipitated from transfected HEK293T cells and the bound endogenous H2B was detected using anti-H2B antibody.</p